Translational_Unit

Part:BBa_K4310013:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


enhanced expression of eGFP by cold stress


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 85
    Illegal PstI site found at 944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3067
    Illegal PstI site found at 85
    Illegal PstI site found at 944
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 85
    Illegal PstI site found at 944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 85
    Illegal PstI site found at 944
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3998
    Illegal BsaI.rc site found at 1727
    Illegal BsaI.rc site found at 4277
    Illegal SapI site found at 1
    Illegal SapI site found at 1254
    Illegal SapI.rc site found at 1239
    Illegal SapI.rc site found at 1288


Design Notes

pCnAFP: BBa_K4310004 Kozak sequence: BBa_K4310003 eGFP: BBa_K431006 Tnos: BBa_J428082 from distribution 2022


Source

To make compatible to our assembly, Tnos are amplified by overhang primers.


References